This article is specifically for gynaecologist and post graduates in life sciences. We taking in a view of this gadget from government of India from December 20, 2021. It is Assisted Reproduction Technology Regulation which came from 2021 and with its ruling came on June 7, 2022 regarding the eligibility criteria of ART 2 clinic, level 2 clinic.

With ART level 2 clinic you need a gynaecologist and you need an embryologist. For making this embryo gynaecologist compatible to start independent ART level 2 clinic that is IVF centre, they need at least 50 ovum pick up procedures under supervision of trained ART specialist.

With this we are starting Fellowship in IVF course where we will give hands on to the person of at for at least 50 ovum pick up along with detail in evaluation of infertile couple, management of IVF, follicular monitoring, ovum pick up, embryo transfer, IUI and diagnostic Hysteroscopy. As we are doing lot of reproductive genetics work also though the person will be exposed to the foetal medicine and genetics also. This course will be for 1 year and will be commencing from April 2026.

We are going to take very few students for a year, they need an eligibility criteria. As we are doing in house embryology, so for post graduate in life sciences and biotechnology which is the most common person will be taking the course for or the career for by this embryology, they also need 1 year onsite full-time clinical embryology.

One such institution is Medline Academics. With the pandemic striking hard, it was almost impossible to attend lectures in a classroom setup, but Medline Academics made it possible with its eLearning mode and hybrid format where the theory is often covered online, and once the midterm examinations are conducted, they will be scheduled for the simulation training and the clinical postings. This institution also offers best IVF fellowship in India unlike any other institution. Besides, the fellowship program here is university affiliated.

So separately we are also starting a 1-year fellowship programme for embryology especially for post graduate life sciences students who are searching for their job as an embryology they want to become an embryology. Both these courses will be under Padma Shri Prof. Dr. Kamini A. Rao, and it will encompass all the details of embryology and reproductive medicine and we will try to give hands on ICSI procedure so that the person can become competent enough to do an independent embryology after finishing this course. So, these 2 courses are we are planning to commence from April 2026, whosoever wants to join as an embryology course for embryology course or for reproductive medicine fellowship course ART fellowship course can contact us and as we are doing regularly every day almost 2-3 IVF.

So, there will be an immense exposure how to run an IVF centre, how to maintain the lab equipment’s, to learn the greater techniques or finer techniques in the field of embryology and reproductive medicine.

But today we will learn IVF procedure step by step in this blog.

What is IVF and when do we do it?

IVF is done when male factor infertility is very severe. Very severe means the number of uteruses is very less. Or female factor infertility like problem in tubes, eggs are there, polycystic ovary is there, eggs are not coming out rr our earlier treatments like small treatments like IUI etc. have failed. Then couple needs IVF.

Basically, IVF is also known as test tube baby. Now we will learn IVF process step by step. Whenever we start IVF, there is a certain preparatory phase.

We devote this preparatory phase when we do female and male investigations. And optimise their body. In male investigations specifically, we do semen analysis and semen culture.

To see that there is no infection. Because infection is not a good thing for our IVF. Infection reduces the quality of IVF and babies.

 In female partner, we do test of preparatory phase. We optimise general well-being, vaginal cultures and other tests. And we give supplements.

So that next cycle when we start IVF, the body is in an optimal stage.

Now we will learn IVF procedure step by step. To start IVF, we have to start from 2nd day.

In 2nd day, we do investigations of female. Basically, we do a baseline enteral follicular count. Which is an ultrasound test, a TBS ultrasound.

In baseline scan, the most important thing we see is that how many eggs are there. How is the lining? And there is no problem in ultrasound. There is no problem in uterus.

Suppose your eggs are less. Then the same cohort of eggs will increase. If 10, then 10 will increase.

If 5, then 5 will increase. If 20, then 20 will increase. It is not that we can put extra number of eggs in IVF.

But yes, the number of small eggs, the number of enteral follicles. We try to increase all of them. Your injections start from 2nd day.

Injections are usually done in the stomach. There is no pain in injections. It is a very common question that IVF injections are very painful.

So, it is not at all. IVF injections are generally done by the patient himself. There is a very small syringe and the injection starts.

After that, again we call the patient for ultrasound monitoring on 5th and 6th day. On 5th and 6th day, we will see how many large follicles are there in the ultrasound. How is its growth? Was our dosing appropriate or not? And most important, according to the size, we have to add an antagonist injection, i.e. another type of injection.

These injections usually run for 7 to 10 days. In the meantime, we do ultrasound monitoring. Either the alternate day patient has to come or daily monitoring is done.

The injections that we apply for IVF infertility, usually after 7 to 10 days of injections, after ovarian stimulation, the eggs come in an appropriate size. And after a particular egg size of 18 mm, we plan the trigger injection. Trigger injection is given for ovarian maturation, for the maturation of eggs.

And along with this trigger injection, our time matches when we have to pick up the egg. Specifically, 34 to 36 hours after trigger injection, this is the time of 2 hours when we have to remove the eggs. The egg pickup procedure is a single time procedure when you feel unconscious in the entire IVF cycle.

Now eggs and semen, both the samples are in our lab. And now the process of fertilisation takes place. Fertilisation is done in two ways.

One is the conventional IVF method and the other is ICSI. ICSI is a particular process where we take a single egg and inject sperm in it. After these two methods, now our embryos will start growing.

And we will monitor them time to time. We will monitor which embryos are growing well, which are getting arrested. Usually, we do not take arrested embryos.

And we take good grade embryos till day 5 and day 3. After this, now we have babies prepared. According to the age of the babies, embryo transfer takes place. Embryo transfer is done in two types.

One is fresh embryo transfer and second is frozen embryo transfer. Fresh embryo transfer is done on the 3rd or 5th day of the ovum pickup cycle. We transfer the embryos to the uterus.

For this, we do some hormonal tests on the day of the trigger. Or we check the lining of the endometrial. If it is proper, then this transfer is possible.

In some cases, like ovarian hyperstimulation, there is a specific frozen transfer. Or if the first eye wave fails, then the next transfer will be frozen embryo transfer. Because at the stage of day 3, the embryos usually freeze for further future use.

Frozen embryo transfer is a new cycle. On the 2nd or 3rd day, the scan begins. Tablets are used to check the lining of the embryos.

Usually, only tablets are used for 10 to 12 days. Then injections are added. Accordingly, we transfer the embryo of day 3 cleavage stage or day 5 blast stage to the female's uterus.

The important thing is that the process of embryo transfer is completely painless. And there is no need for anaesthesia. Patients lie down completely comfortably.

And keep watching on the ultrasound machine that the embryos are being transferred to the uterus. It is an absolutely pain-free process. After this, we give injections, vaginal tablets, and support of progesterone.

This support will usually last for 12 to 14 days. And then you will do the pregnancy test. In the pregnancy test, it is found whether the pregnancy has come or not. Whether the happiness has come to your home.

Looking for IVF Centres in Bangalore? At Dr. Kamini Rao Hospitals, the process of evaluating male fertility is much more than just looking at numbers on a test report. The hospital follows strict, well-established procedures for semen analysis, which are carried out by experienced embryologists using state-of-the-art laboratory equipment. This ensures that the results are not only accurate but also dependable. What makes this approach even more special is that each report is carefully reviewed with the individual patient in mind. Factors such as hormone levels, lifestyle choices, past medical history, and the couple's fertility goals are all taken into account during the interpretation of the results. Whether the findings are within normal ranges or indicate some abnormalities, patients are given clear and understandable advice on what to do next. This may include making changes to their lifestyle, seeking medical treatment, or exploring more advanced options like intrauterine insemination (IUI), in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI). With years of experience and leadership in the field of reproductive medicine, Dr. Kamini Rao Hospitals provides complete, science-backed care to couples at every stage of their path to parenthood.